LOCUS       pLV-[hsa-miR-539]        9132 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7053..7913
                     /label=Amp(R)
     promoter        8114..8654
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5658..5892
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6467..6922)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5964..6094
                     /label=SV40 pA
     misc_feature    6095..6308
                     /label=SV40 ori
     misc_feature    4979..5575
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3597
                     /label=intron
     CDS             3644..4969
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3387
                     /label=hsa-mir-539 precursor
BASE COUNT     2158 a      2365 c      2485 g      2124 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct gtgtgtgcat 
     3121 gagcgagtgt gtgactgggt ttgccacgaa gcagtcacca tctaaccttg agccaaagac 
     3181 ctggaggtgg tggtccgtgc tgctgtttca tacttgagga gaaattatcc ttggtgtgtt 
     3241 cgctttattt atgatgaatc atacaaggac aatttctttt tgagtatcaa atcttgcctc 
     3301 ggcagacttc ctggacgcca tgacaagttc tgtaaaggga tgcacgcatc agcccttccc 
     3361 aggggctcac gcctgggtgg ggatgaggct agctcgagct tttggagtac gtcgtcttta 
     3421 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa 
     3481 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga 
     3541 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg 
     3601 tcgtgaaaac tacccctcta gagtcgagct accggtcgcc accatggtga gcaagggcga 
     3661 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 
     3721 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 
     3781 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 
     3841 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 
     3901 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 
     3961 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 
     4021 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 
     4081 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 
     4141 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 
     4201 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 
     4261 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 
     4321 ctccaccggc ggcatggacg agctgtacaa ggacccaccg gtcgccacca tgaccgagta 
     4381 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 
     4441 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 
     4501 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 
     4561 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 
     4621 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 
     4681 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 
     4741 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 
     4801 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 
     4861 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgagtg 
     4921 cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc ggtgcctgag cggccgcgtc 
     4981 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 
     5041 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 
     5101 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 
     5161 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 
     5221 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 
     5281 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 
     5341 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg 
     5401 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 
     5461 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 
     5521 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt 
     5581 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 
     5641 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt 
     5701 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 
     5761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 
     5821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 
     5881 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga 
     5941 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa 
     6001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     6061 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc 
     6121 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     6181 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 
     6241 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac 
     6301 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 
     6361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 
     6421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 
     6481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 
     6541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 
     6601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 
     6661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 
     6721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 
     6781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 
     6841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 
     6901 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 
     6961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 
     7021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 
     7081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 
     7141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 
     7201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 
     7261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 
     7321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 
     7381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 
     7441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 
     7501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 
     7561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 
     7621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 
     7681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 
     7741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 
     7801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 
     7861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 
     7921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 
     7981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 
     8041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 
     8101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 
     8161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 
     8221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 
     8281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 
     8341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 
     8401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 
     8461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 
     8521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 
     8581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 
     8641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 
     8701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 
     8761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 
     8821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 
     8881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 
     8941 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 
     9001 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 
     9061 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa 
     9121 agctggagct gc 
//
